DIACETYL FLUORESCEIN

DIACETYL FLUORESCEIN
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Product code: RXSOL-60-6604-420

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 Fluorescein diacetate (FDA) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product.

Application

To prepare a FDA stock solution 5 mg of fluorescein diacetate is dissolved in 1 ml acetone.
2,5 ml cell culture medium is cooled on ice.
50 ml of the FDA stock solution is added and this diluted FDA solution is stored on ice.

Procedure

Fluorescence-based live-dead assays can be used to evaluate the viability of mammalian cells. ... In this Application Note we present a staining protocol using fluorescein diacetate (FDA) and propidium iodide (PI), which stain viable cells and dead cells, respectively.

Other names

DIACETYL FLUORESCEIN

Supply locations

[DIACETYL FLUORESCEIN] manufacturers, suppliers, exporters in Mumbai, Gandhidham, Kolkata, Varanasi, Visakhapatnam, Chennai, Fujairah, Dubai, Canada BC, Barka, Sohar, Muscat, Oman.
Lab chemicals manufacturers, suppliers, exporters in India, UAE Middle East, Barka, Sohar, Muscat, Oman, Canada.
 
[DIACETYL FLUORESCEIN] is available in small packing as well as in bulk. Buy premium quality [DIACETYL FLUORESCEIN] and other lab chemicals from one of the most trusted brands.

Note

Dilute the fluorescein solution by add- ing enough distilled or deionized water to reach the 500-mL mark on the beaker. Stir. Note: Prepare 1% fluorescein solution by dissolving 1 g of fluorescein in 100-mL distilled water.